https://doi.org/10.3390/antibiotics12010177
“EO samples were analysed and the relative peak areas for individual constituents were averaged. The relative percentages were determined using a ThermoQuest GC-Trace gas-chromatograph equipped with a FID detector and a Varian FactorFour VF-5ms poly-5% phenyl-95%-dimethyl-siloxane bonded phase column (i.d., 0.25 mm; length, 30 m; film thickness, 0.25 µm). Operating conditions were as follows: injector temperature 280 °C; FID temperature 300 °C, carrier gas (Helium) flow rate 1 mL/min and split ratio 1:50. Oven temperature was initially 55 °C and then raised to 90 °C at a rate of 1 °C/min, then raised to 250 °C at a rate of 10 °C/min and finally held at that temperature for 15 min. Each sample was dissolved in CH2Cl2 (1:100 v/v) and 1 μL was injected. The percentage composition of the EOs was computed by the normalisation method from the GC peak areas, calculated by means of three injections from each EO, without using correction factors.”