Analysis of fatty acid profile by gas chromatography (GC-FID)

“The fatty acid methyl esters (FAMEs) were quantified by a gas chromatography method using a J & W Scientific HP-88 series 100 m × 0.25 mm × 0.20 μm fused silica capillary column (Agilent Tech. Inc., St. Clara, CA, USA) and flame-ionization detector (FID) from Agilent Tech. Hemp oils were saponified with 0.5 M KOH in methanol. Transesterification of fatty acids with the BF3 (boron trifluoride) solution in methanol was carried out using the official American Oil Chemists’ Society (AOCS) Ce 2–66 method [54]. We used a 7890 A series gas chromatograph (Agilent Tech. Inc., St. Clara, CA, USA) at an injection volume of 1.0 mL and a split ratio of 1/50. Helium was used as the carrier gas at a head pressure of 2.0 mL/min at a constant flow. Air, hydrogen, and helium make-up gas flow rates by FID (Agilent Tech. Inc., St. Clara, CA, USA) were 450, 40, and 30 mL/min, respectively. The detector and injector temperatures were chosen as 280 °C and 250 °C, respectively. The initial column temperature of 120 °C was held for 1 min, increased to 175 °C at 10 °C/min and then held for 10 min. It was then increased to 210 °C at 5 °C/min, held for 5 min, increased to 230 °C at a rate of 5 °C/min, and maintained for 5 min. The conditions for chromatographic separation conformed with the procedure described by Wołoszyn et al. [55].”

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