https://doi.org/10.3390/plants12010039
“Both samples were diluted (1/100, v/v, EO/DCM) and analyzed using a Thermo Scientific Gas Chromatograph (TRACE 1300 Series) coupled to a Single Quadrupole Mass Spectrometer (ISQ 7000 Series). A non-polar DB-5 ms column of 0.25 mm × 30 m, a thickness of 0.25 µm (5%-phenyl-methylpolyxilosane) was used. For each run, 1 µL of the EO dilutions were injected with a split ratio of 1:40. The equipment operated with electronic ionization (70 eV). Methane was used as a carrier gas at 1 mL/min in constant flow mode. The initial oven temperature was 60 °C, following a gradient until 230 °C was reached. Each run lasted 58 min. The Retention Index was calculated using alkanes from C9 to C10, which were previously injected under the same conditions. The compounds were identified through a comparison between the CRI and reference literature [23].”