Identification of Active Compounds Using GC-MS

“Compounds were identified using the gas chromatography–mass spectrometry (GC-MS) method. In GC-MS, the analyte is separated using gas chromatography, and its identity is confirmed using mass spectrophotometry techniques [70]. The sample was first dissolved in 50 µL of organic solvent. A sample of 10 µL was injected in the injection port at 290 °C. The volatilized sample was carried by helium at a flow rate of 1 mL/min through a gas chromatography column. The temperature at the time of injection was 80 °C and increased by 10 °C per minute, with a final temperature of 300 °C (43 min) [70]. The detection of compounds took place in a mass spectrometry system with the mechanism of crashing or bombarding compounds with electrons to form ionized molecules and record fragmentation patterns. The fragmented mass components were compared with WILEY and NIST standard reference data, as indicated by the percentage similarity index (SI) [38].”

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