HS-SPME-GC–MS analysis

“Brown rice (8 g) and 10 μL 2-octanol (0.1 mg/mL, 99.99 %) were put in a 20 mL headspace container, which was then placed in a 70 °C water bath for 30 min. The volatile compounds were extracted by exposing the fiber (50/30 μm DVB/CAR/PDMS, Supelco) to the samples for 70 min. GC–MS (QP2010-plus, Shimadzu Co., Japan) was equipped with a Rxi-5 MS column (30.0 m × 0.25 mm, 0.25 μm). Following these steps, the chromatographic column was programmed to increase from the initial temperature of 45 °C to 250 °C at a rate of 5 °C/min. Both processes are held for 5 min. Helium (purity of 99.99 %) was used as a carrier gas and its flow rate was set at 1 mL/min. The temperatures of the ion source and the interface are 200 °C and 250 °C, respectively. The full scan is carried out at a speed of 1666 amu/s, and the scanning range is 45–500 amu.

Qualitative analysis of volatile compounds was accomplished by comparison with the standard atlas libraries (NIST08 and NIST08s). The ratio of peak areas was used for quantitative analysis and the calculation formula is as follows:C=S1×m2S2×m1×1000Where C is the content of volatile compounds (μg/kg), S1 is the chromatographic peak area of the compound to be tested, S2 is the chromatographic peak area of the internal standard, m1 is the sample mass (g), and m2 is the internal standard mass (μg). The results were displayed as mean ± standard deviation of triplicate.”

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