GC-MS analysis of organic fractions of Chrozophora tinctoria

“Gas Chromatography-Mass Spectrometry (GC-MS)

To identify the different phytochemicals present in the ethyl acetate fraction of Chrozophora tinctoria (EAFCT) and dichloromethane fraction of Chrozophora tinctoria (DCMFCT), the sample was subjected to GC-MS. The sample was checked using Thermo Scientific (DSQ-II) GC. The gas chromatography (GC) was furnished with a 30 meter lengthy TR-5MS capillary column, 0.25µm thick film, and 0.25mm of internal diameter. Helium (He) was used as carrier gas with 1ml/min of flow rate. The injection device was run in a split mode having 2500C. 1µl sample of the volume was administered with a beginning oven temperature of 50°C and maintained for 2 minutes then the temperature was raised to 150 °C with a flow rate of 08°C/min. After this with an interval of 15°C/min temperature was elevated to 300°C and controlled for 5 minutes (Alam et al., 2014).”


“Figure 1 GC-MS chromatogram of the ethyl acetate fraction of Chrozophora tinctoria (EAFCT) the numbers showed the retention times of various compounds.”

“The EAFCT was checked for phytochemicals, the EAFCT displayed several peaks, which exhibit different bioactive compounds present in the sample (Figure 1). These peaks were matched with the database of the spectrum of known components present in the GC-MS library.”

“GC-MS analysis of EAFCT showed the presence of many bioactive compounds at different retention times (min). about 9 compounds were identified. These compound are Hexadecanoic acid, ethyl ester (0.07%), Hexadecanoic acid, methyl ester (0.06%), 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (0.03%), Pentadecanoic acid, 14-methyl-, methyl ester (0.02%), 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (0.01%), Imipramine (0.01%), D-Tyrosine, 3-hydroxy- (0.01%), 1-Tricosanol (0.01%) and Nonanoic acid, 9-(0-propylphenyl)- methyl ester (0.01%) Table 1.”

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