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Essential Oil Chemical Component Acquisition – GC/MS

https://doi.org/10.3390/en15218092

“The confirmation of the phytochemical constituents of F. hispida EOs was performed using an Agilent gas chromatograph–mass spectrometer (GC–MS) (7890-5975C). Briefly, the chromatography conditions were as follows: chromatographic column, HP-5MS (30 m × 0.25 mm × 0.25 μm); injector temperature, 270 °C; carrier gas, helium at a flow rate of 1.10 mL/min; temperature-rising program, initial oven temperature, 60 °C, increasing by 7 °C/min to 220 °C and held stable for 6 min, and then increasing by 10 °C/min to 280 °C and held stable for 6 min. The mass spectrometer conditions were as follows: EI: 70 eV, 230 °C, the mass scan range of 20–450 Da, and an acquisition frequency of 2. The quadrupole temperature was 150 °C, and 0.5 μL samples were injected.

The EOs and the n-alkane (C7–C30) were analyzed under the same GC conditions. The data processing was carried out by Agilent MassHunter Qualitative Analysis 10.0 program, and the relative abundance of each compound in the EOs was determined by the peak area normalization. The identification of the essential oil components was carried out by calculating their retention indices (RI) in a temperature-dependent programmed condition and was compared with the spectral library (NIST/EPA/NIH 2020).”

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