https://doi.org/10.1038/s41598-022-24268-5
“Gas chromatography coupled to mass spectrometry (GC–MS) on a gas chromatograph HP 7890 (II) and HP 5975 mass spectrometer (Agilent Technologies, Palo Alto, CA, USA ) with an electron impact ionization of 70 eV, HP 5 MS column with dimensions of 30 m length; 0.25 mm diameter and 0.25 µm film thickness was operated at a programmed temperature of 40–280 °C at a rate of 5 °C/min. Helium was used as a carrier at a flow rate of 1.2 mL/min.
Quantitative analyses were obtained using a Hewlett–Packard 7890 chromatograph equipped with a flame ionization detector (FID) and an electronic pressure control injector. An HP Innowax capillary column (polyethylene glycol, 30 m × 0.25 mm, 0.25 μm film thickness) (Agilent) was used at carrier gas N2 with a flow rate of 1.6 mL/min and a split ratio of 1:60. The column temperature was programmed at 35 °C for 10 min, then heated to 205 °C at a rate of 2 °C/min, and finally kept constant at 205 °C for 10 min. Injector and detector temperatures were held at 250 and 300 °C, respectively.
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